首頁 > 植物生物學(xué) > 植物染色 > GUS染色
GUS染色試劑盒(貨號:RTU4032)
● 產(chǎn)品組成:
組分貨號 |
名稱 |
規(guī)格 |
貯存 |
運輸 |
RTU4032-01 |
X-gluc |
粉末 |
-20℃ |
RT |
RTU4032-02 |
X-gluc溶解液 |
1 ml |
-20℃ |
RT |
RTU4032-03 |
GUS染色緩沖液 |
50 ml |
4℃ |
RT |
|
說明書 |
一份 |
|
|
● 產(chǎn)品簡介:
gus(β-glucuronidase,β-D- 葡萄糖苷酸酶)基因是目前常用的一種報告基因,其表達(dá)產(chǎn)物β-葡萄糖苷酸酶 (GUS)是一種水解酶,能催化許多β-葡萄糖苷酯類物質(zhì)的水解,它可以將5-溴-4-氯-3-吲哚-β-葡萄糖苷酸酯(x-gluc)分解為藍(lán)色的物質(zhì),其檢測方法簡單、快速、靈敏、穩(wěn)定,且背景活性低。因為絕大多數(shù)植物細(xì)胞內(nèi)不存在內(nèi)源的GUS活性,因此gus基因廣泛用作轉(zhuǎn)基因植物的報告基因,尤其是在研究外源基因瞬時表達(dá)的轉(zhuǎn)化實驗中廣泛應(yīng)用。
該試劑盒包含GUS染色的全部試劑,使用方便,只需將配制好的X-gluc溶液和緩沖液按照比例混合即配成GUS染色液。該試劑盒可以配制50ml GUS染色液。
● 貯存和效期:
未配制的試劑盒按照溫度貯存,有效期2年。
配制好的X-gluc溶液-20℃避光保存30天,長期-80℃保存;GUS染色緩沖液 4℃貯存;GUS染色工作液最好現(xiàn)用現(xiàn)配,短期貯存可以-20℃保存2-3天
● 使用說明:
一、X-gluc溶液(50×)配制:
吸取1ml X-gluc溶解液加入到X-gluc管中,徹底混勻,至粉末完全溶解,即配成X-gluc溶液(50×),該溶液-20℃避光保存。
注:X-gluc溶液穩(wěn)定性較差,-20℃避光保存30天左右溶液保持無色,長期保存建議按照需要分裝到棕色管里,-80℃避光保存。正常的X-gluc溶液顏色為無色,如果溶液變?yōu)榧t色或棕色,表明溶液失效。
二. GUS染色工作液配制:
|
GUS染色工作液 配制量 |
||
|
1 ml |
5 ml |
10 ml |
X-gluc溶液(50×) |
20 μl |
100 μl |
200 μl |
GUS染色緩沖液 |
1 ml |
5 ml |
10 ml |
注:GUS染色工作液最好現(xiàn)用現(xiàn)配,短期貯存可以-20℃保存2-3天。
三. GUS染色步驟:
1.取材:將葉片、花瓣、根莖等組織剪成小片,放于1.5ml離心管中。
注:用于染色的植物材料的制備方法要因涉及的特定組織和器官的不同而異。例如,擬南芥的根、花和葉片以及煙草幼苗的根就可以不作任何預(yù)處理而直接染色。但是像煙草和馬鈴薯這些植物的莖和葉就必須在染色前切成薄片(1-3mm)。當(dāng)操作大的組織和樣品時,可以選用真空滲入法來幫助底物滲入細(xì)胞。
2.染色:加入適量配制好的GUS染色工作液至完全覆蓋材料;錫箔紙包好后搖床搖動10分鐘或更長時間至材料出現(xiàn)藍(lán)色。注意要做陰性和陽性對照實驗。
3.洗脫:將材料轉(zhuǎn)入無水乙醇或80%丙酮中脫色2-3次,至陰性對照材料為白色。GUS染色陽性的藍(lán)色斑點很穩(wěn)定,在酒精中不褪色。
4.觀察:一般染色后,GUS染色陽性的藍(lán)色斑點肉眼就能看到。但是有些材料可能藍(lán)色斑點很細(xì)微,要在顯微鏡下才能看到。
1 GUS最原始文獻(xiàn)
GUS fusions: ,B-glucuronidase as a sensitive and versatile gene fusion marker in higher plants. The EMBO Journal vol.6 no. 13 pp.3901 -3907, 1987
使用RTU4032 GUS染色試劑盒發(fā)表部分文章列表(71篇)
1. [IF=6.456] A PIF1/PIF3-HY5-BBX23 Transcription Factor Cascade Affects Photomorphogenesis.
Author: Xinyu Zhang, Fangfang Shang, Junling Huai, Gang Xu, Weijiang Tang, Yanjun Jing,Rongchen g Lin.
Journal: Plant Physiology. 2017,Vol. 174, 2487–2500.
Institution: Institute of Botany,Chinese Academy of Sciences,Beijing,China
Paper link:http://www.plantphysiol.org/content/174/4/2487
2. [IF=6.17] PICKLE chromatin remodeling factorcontrols thermosensory hypocotyl growth of Arabidopsis.
Author: Zha P, Jing YJ, Xu, G, Lin RC
Journal: Plant, Cell and Environment.2017,Vol40, 2426–36.
Institution: Institute of Botany,Chinese Academy of Sciences,Beijing,China
Paper link:http://onlinelibrary.wiley.com/doi/10.1111/pce.13049/full
3. [IF=4.3] The Wheat Bax Inhibitor-1 Protein Interacts withan Aquaporin TaPIP1 and Enhances Disease Resistance in Arabidopsis.
Author: Pan-Pan Lu,Tai-Fei Yu,Wei-Jun Zheng, Ming Chen, Yong-Bin Zhou,Jun Chen, You-ZhiMa, Ya-Jun Xi and Zhao-Shi Xu.
Journal: Frontiers in Plant Science.2018,Vol.9
Institution:College of Agronomy, Northwest A&F University;
Paper link:https://www.frontiersin.org/articles/10.3389/fpls.2018.00020/full
4. [IF=3.33] Expression and functional analysis of two PsbS genes in bamboo (Phyllostachys edulis).
Author: Yongfeng Lou,Huayu Sun,Sining Wang,Hao Xu,Lichao Li,Hansheng Zhao,Zhimin Gao.
Journal: Pysiol Plant. 2018
Institution: International Center for Bamboo andRattan, Beijing,China
Paper link:http://onlinelibrary.wiley.com/doi/10.1111/ppl.12690/full
5. [IF=3.154] MultiplePesticides Detoxification Function of Maize (Zea mays) GST34.
Author: Dongzhi Li, Li Xu, Sen Pang, Zhiqian Liu, Weisong Zhao, Chengju Wang.
Journal: J. Agric. Food Chem. 2017 ,65 (9),pp 1847–1853
Institution:College of Science, China Agricultural University, Beijing,China
Paper link:http://pubs.acs.org/doi/abs/10.1021%2Facs.jafc.7b00057
6.[IF=2.87] The bambooaquaporin gene PeTIP4;1–1 confers drought and salinity tolerance in transgenicArabidopsis.
Author: Huayu Sun, Lichao Li, Yongfeng Lou, Hansheng Zhao, Yihong Yang, Sining Wang, Zhimin Gao.
Journal: Plant Cell Rep 2017,Vol36, 4, 597–609
Institution: International Center for Bambooand Rattan, Beijing,China
Paper link:https://link.springer.com/article/10.1007/s00299-017-2106-3
7.[IF=2.236] Characterizationand Primary Functional Analysis of a Bamboo ZEP Gene from Phyllostachys edulis.
Author: Yongfeng Lou, Huayu Sun, Lichao Li,Hansheng Zhao, Zhimin Gao.
Journal: DNA and Cell Biology. 2017,Vol 36,9,1-12
Institution: International Center for Bambooand Rattan, Beijing,China
Paper link:http://online.liebertpub.com/doi/abs/10.1089/dna.2017.3705
8.[IF=3.687] Expression Pattern and Function Analysis of AtPPRT1, a NovelNegative Regulator in ABAand Drought Stress Responses in Arabidopsis
Author: Linsen Pei, Lu Peng, Xia Wan, Jie Xiong, Zhibin Liu, Xufeng Li, Yi Yang andJianmei Wang.
Journal: Int. J. Mol. Sci. 2019, 20, 394
Institution: College of Life Sciences, Sichuan University
Paper link:https://www.mdpi.com/1422-0067/20/2/394
9. [IF=3.93] Wheat Bax Inhibitor-1 interacts with TaFKBP62 and mediates responseto heat stress
Author: Pan-Pan Lu, Wei-Jun Zheng, Chang-TaoWang, Wen-Yan Shi, Jin-Dong Fu, Ming Chen, Jun Chen,Yong-Bin Zhou, Ya-Jun Xi andZhao-Shi Xu.
Journal: BMC Plant Biology 2018, 18,259
Institution:Institute of Crop Science, Chinese Academy of Agricultural Sciences
Paper link: https://bmcplantbiol.biomedcentral.com/articles/10.1186/s12870-018-1485-0
10. [IF=5.415] SpHMA1 is a Chloroplast Cadmium Exporter Protecting Photochemical Reactions in the Cd Hyperaccumulator Sedum plumbizincicola
Author: Haixia Zhao Liangsheng Wang Fang‐Jie Zhao Longhua Wu Anna Liu Wenzhong Xu.
Journal: Plant, Cell and Environment 12 October 2018
Institution: Institute of Botany, Chinese Academy of Sciences,Beijing,China
Paper link: https://onlinelibrary.wiley.com/doi/abs/10.1111/pce.13456
11. [IF=8.827] SEUSS and PIF4 Coordinately Regulate Light and Temperature Signaling Pathways to Control Plant Growth.
Author: Junling Huai, Xinyu Zhang, Jialong Li, Tingting Ma, Ping Zha, Yanjun Jing,Rongcheng Lin.
Journal: Molecular Plant 2018,11,928-942
Institution: Institute of Botany, Chinese Academy of Sciences,Beijing, China
Paper link: https://doi.org/10.1016/j.molp.2018.04.005
12. [IF=3.825] AtARRE, an E3 ubiquitin ligase, negatively regulates ABA signaling in Arabidopsis thaliana.
Author: Boya Wang,Chuzhe Li,Xiangge Kong, Ying Li, Zhibin Liu, Jianmei Wang, Xufeng Li, Yi Yang.
Journal: Plant Cell Reports 37, 1269–1278 (2018)
Institution: College of Life Sciences, Sichuan University
Paper link: https://doi.org/10.1007/s00299-018-2311-8
13. [IF=6.902] OsDER1 is an ER-associated protein degradation factor that responds to ER stress. Author: Dandan Qian, Guoqiang Chen, Lihong Tian, Le Qing Qu.
Journal: Plant Physiol 2018 178,402-412
Institution: Institute of Botany, Chinese Academy of Sciences
Paper link: https://doi.org/10.1104/pp.18.00375
14. [IF 3.93] Wheat Bax Inhibitor-1 interacts with TaFKBP62 and mediates response to heat stress. Author: Pan-Pan Lu, Wei-Jun Zheng, Chang-Tao, Wang, Zhaoshi Xu.
Journal: BMC Plant Biology 18, 259 (2018)
Institution: Institute of Crop Science, Chinese Academy of Agricultural Sciences
Paper link: https://doi.org/10.1186/s12870-018-1485-0
15. [IF 5.415] The chromatin-remodelling factor PICKLE interacts with CONSTANS to promote flowering in Arabidopsis.
Author: Yanjun Jing, Qiang Guo, Ping Zha, Rongcheng Lin.
Journal: Plant, Cell and Environment 2019
Institution: Institute of Botany, Chinese Academy of Sciences, Beijing
Paper link: https://doi.org/10.1111/pce.13557
16. [IF 5.415] The Arabidopsis defensin gene AtPDF2.5 mediates cadmium tolerance and accumulation.
Author: Jin-Song Luo, Yong Yang, Tianyu Gu, Zhimin Wu, Zhenhua Zhang.
Journal: Plant, Cell and Environment 2019 May
Institution:Hunan Agricultural University
Paper link: https://doi.org/10.1111/pce.13592
17. [IF 3.517] In Silico Genome-Wide Analysis of the Pear (Pyrus bretschneideri) KNOX Family and the Functional Characterization.
Author: Xi Cheng, Yongping Cai.
Journal: Frontiers in Genetics 2019,10 Article 632
Institution:Anhui Agricultural University
Paper link: https://doi.org/10.3389/fgene.2019.00632
18.[IF 3.929] Chloroplast-Localized Protoporphyrinogen IX Oxidase1 Is Involved in the Mitotic Cell Cycle in Arabidopsis.
Author: Jialong Li, Fan Zhang, Yuhong Li, Weicai Yang, Rongcheng Lin
Journal: Plant and Cell Physiology 2019
Institution: Institute of Botany, Chinese Academy of Sciences, Beijing, China
Paper link: https://doi.org/10.1093/pcp/pcz135
19. [IF 3.259] Functional characterization of three MATE genes in relation to aluminum-induced citrate efflux from soybean root.
Author: Ying Zhou, Zhengbiao Wang, Li Gong, Anle Chen, Ning Liu, Sha Li, Haoran Sun, Zhenming Yang, Jiangfeng You
Journal: Plant Soil 443, 121–138 (2019)
Institution:College of Plant Science, Jilin University
Paper link: https://doi.org/10.1007/s11104-019-04192-w
20. [IF 10.8] bHLH121 Functions as a Direct Link that Facilitates the Activation of FIT by bHLH IVc Transcription Factors for Maintaining Fe Homeostasis in Arabidopsis.
Author:RihuaLei, YangLi, YuerongCai, ChenyangLi, MengnaPu, ChengkaiLu, YujieYang, GangLiang.
Journal: Molecular Plant Vol 13, Issue 4, 6 April 2020, 634-649
Institution:Xishuangbanna Tropical Botanical Garden, Chinese Academy of Sciences
Paper link: https://doi.org/10.1016/j.molp.2020.01.006
21. [IF 11.88] A mycorrhizae-like gene regulates stem cell and gametophore development in mosses.
Author: ShuanghuaWang, Jinling Huang
Journal: Nature Communications 2020,11,2030
Institution:Kunming Institute of Botany, Chinese Academy of Sciences
Paper link: https://doi.org/10.1038/s41467-020-15967-6
22. [IF 4.1] Overexpression of a Defensin-Like Gene CAL2 Enhances Cadmium Accumulation in Plants .
Author: Jin-Song Luo,Zhenhua Zhang.
Journal: Frontiers in Plant Science 2020
Institution:Hunan Agricultural University
Paper link: https://doi.org/10.3389/fpls.2020.00217
23. [IF 1.644] AtPPRT1 negatively regulates salt stress response in Arabidopsis seedlings.
Author: Yu Liu, Linsen Pei, Shuya Xiao, Lu Peng, Zhibin Liu, Xufeng Li, Jianmei Wang.
Journal: Plant Signaling & Behavior 2020
Institution:Sichuan University
Paper link: https://doi.org/10.1080/15592324.2020.1732103
24. [IF 3.824] Oryza Sativa Fer-Like Fe Deficiency Induced Transcription Factor (Osfit/Osbhlh156) Interacts With Osiro2 To Regulate Iron Homeostasis.
Author: Gang Liang, Huimin Zhang, Yang Li, Mengna Pu, Yujie Yang, Chenyang Li, Chengkai Lu, Peng Xu, Diqiu Yu.
Journal: J Integr Plant Biol 2020 ,Volume 62 ,Issue 5 ,668–689
Institution:Xishuangbanna Tropical Botanical Garden, Chinese Academy of Sciences
Paper link: https://doi.org/10.1111/jipb.12933
25. [IF暫無] RGG1, involved in the cytokinin regulatory pathway,controls grain size in rice.
Author:Yajun Tao, Jie Yang
Journal: Reaearch Square 2020
Institution:Jiangsu Academy of Agricultural Sciences
Paper link: https://doi.org/10.21203/rs.3.rs-40598/v1
26. [IF 4.0] Analysis of β-d-glucan biosynthetic genes in oat reveals glucan synthesis regulation by light.
Author:Jing Zhang, Lin Yan, Minxuan Liu, Ganggang Guo and Bing Wu
Journal: Annals of Botany 2020 127: 371–380, 2021
Institution:Institute of Crop Science, Chinese Academy of Agricultural Sciences
Paper link: https://academic.oup.com/aob/article-abstract/127/3/371/5934920
27. [IF 1.5] OsHIPP24 is a Copper Metallochaperone Which Affects Rice Growth.
Author:Guoqiang Chen,Shuo Xiong
Journal: Journal of Plant Biology Nov 30 2020
Institution:Institute of Botany, Chinese Academy of Agricultural Sciences
Paper link: https://www.sciencedirect.com/science/article/abs/pii/S0006291X21005581
28. [IF 8.154] Three AP2/ERF family members modulate ?avonoid synthesis by regulating type IV chalcone isomerase in citrus.
Author:Chenning Zhao, Xiaojuan Liu , Qin Gong , Jinping Cao , Wanxia Shen , Xueren Yin , Donald Grierson , Bo Zhang , Changjie Xu , Xian Li , Kunsong Chen , Chongde Sun.
Journal: Plant Biotechnology Journal (2021) 19, pp. 671–688
Institution:Zhejiang University
Paper link: https://onlinelibrary.wiley.com/doi/10.1111/pbi.13494
29. [IF 10.15] JMJ27-mediated histone H3K9 demethylation positively regulates drought-stress responses in Arabidopsis.
Author:Qiongli Wang, Peng Liu, Hua Jing, Xiao Feng Zhou, Bo Zhao, Yuan Li, Jing Bo Jin
Journal: New Phytologist (2021) 01 July 2021
Institution:IBCAS
Paper link: https://nph.onlinelibrary.wiley.com/doi/epdf/10.1111/nph.17593
30. [IF 6.79] Plasma membrane-localized SlSWEET7a and SlSWEET14 regulate sugar transport and storage in tomato fruits.
Author:Xinsheng Zhang, Chaoyang Feng, Manning Wang, Tianlai Li, Xin Liu, and Jing Jiang
Journal: Horticulture Research (2021) 8:186
Institution:College of Horticulture, Shenyang Agricultural University,
Paper link: https://www.nature.com/articles/s41438-021-00624-w
31. [IF 5.75] A Golgi-Localized Sodium/Hydrogen Exchanger Positively Regulates Salt Tolerance by Maintaining Higher K+/Na+ Ratio in Soybean.
Author:Tianjie Sun,Jie Zhang, Jinfeng Cao and Dongmei Wang
Journal: Frontiers in Plant Science (2021) 12:638340
Institution:College of Life Sciences, Hebei Agricultural University
Paper link: https://www.frontiersin.org/articles/10.3389/fpls.2021.638340/full
32. [IF 5.9]Expression Analyses of Soybean VOZ Transcription Factors and the Role of GmVOZ1G in Drought and Salt Stress Tolerance.
Author:Bo Li 1,y, Jia-Cheng Zheng 2,y, Ting-Ting Wang 3,y, Dong-Hong Min 4, Wen-Liang Wei 3,Jun Chen 1, Yong-Bin Zhou 1, Ming Chen 1, Zhao-Shi Xu 1,* and You-Zhi Ma
Journal: International Journal of Molecular Sciences (2020) 21:2177
Institution:Institute of Crop Science, Chinese Academy of Agricultural Sciences
Paper link:https://pubmed.ncbi.nlm.nih.gov/32245276/
33. [IF 7.228]Close arrangement of CARK3 and PMEIL affects ABA‐mediated pollen sterility in Arabidopsis thaliana.
Author:Hsi-Hua Wang, Yao Qiu, Qin Yu, Qian Zhang, Xiaoyi Li, Jianmei Wang,Xufeng Li, Yang Zhang, Yi Yang
Journal: Plant Cell and Environment 2020;43:2699–2711.
Institution:Sichuan University
Paper link:https://onlinelibrary.wiley.com/doi/10.1111/pce.13871
34. [IF 5.92]PDF1.5 Enhances Adaptation to Low Nitrogen Levels and Cadmium Stress.
Author:Zhimin Wu, Dong Liu, Ningyan Yue, Haixing Song, Jinsong Luo,and Zhenhua Zhang
Journal: International Journal of Molecular Sciences 2021, 22, 10455..
Institution:Hunan Agricultural University
Paper link:https://www.mdpi.com/1422-0067/22/19/10455
35. [IF 6.29]Repression of transcription factor AtWRKY47 confers tolerance to boron toxicity in Arabidopsis thaliana.
Author:Yingna Feng, Rui Cui, Yupu Huang, Lei Shi, Sheliang Wang, Fangsen Xu
Journal: Ecotoxicology and Environmental Safety 220(2021)112406
Institution:Huazhong Agricultural University
Paper link:https://www.sciencedirect.com/science/article/pii/S0147651321005182?via%3Dihub
36. [IF 6.79]PpINH1, an invertase inhibitor, interacts with vacuolar invertase PpVIN2 in regulating the chilling tolerance of peach fruit.
Author:Yingna Feng, Rui Cui, Yupu Huang, Lei Shi, Sheliang Wang, Fangsen Xu
Journal:Horticulture Research ( 2020) 7:168
Institution:Ningbo University
Paper link:https://www.nature.com/articles/s41438-020-00389-8
37. [IF 8.154]Transcription factor BnaA9.WRKY47 contributes to the adaptation of Brassica napus to low boron stress by up-regulating the boric acid channel gene BnaA3.NIP5;1.
Author:Yingna Feng, Rui Cui, Sheliang Wang, Mingliang He, Yingpeng Hua, Lei Shi, Xiangsheng Ye and Fangsen Xu
Journal:Plant Biotechnology Journal (2020) 18, pp. 1241–1254
Institution:Huazhong Agricultural University
Paper link:https://onlinelibrary.wiley.com/doi/10.1111/pbi.13288
38. [2021IF 5.92]An Efficient Marker Gene Excision Strategy Based on CRISPR/Cas9-Mediated Homology-Directed Repair in Rice.
Author:Jiantao Tan, Yaxi Wang, Shuifu Chen, Zhansheng Lin, Yanchang Zhao, Yang Xue, Yuyu Luo,Yao-Guang Liu, Qinlong Zhu
Journal:International Journal of Molecular Sciences,2022, 23(3), 1588
Institution:South China Agricultural University
Paper link:https://www.mdpi.com/1422-0067/23/3/1588
39. [2021IF 3.935]Identification and Functional Analysis of the CgNAC043 Gene Involved in Lignin Synthesis from Citrus grandis “San Hong”.
Author:Xiaoting Li, Naiyu Wang, Wenqin She, Zhixiong Guo, Heli Pan, Yuan Yu , Jianwen Ye,Dongming Pan,Tengfei Pan
Journal:Plants,2022, 11(3), 403
Institution:Fujian Agriculture and Forestry University
Paper link:https://www.mdpi.com/2223-7747/11/3/403/htm
40. [2021IF 2.4] Identification of an anther-specific promoter from a male sterile AB line in Chinese cabbage (Brassica rapa L. ssp. pekinensis)
Author:Ying Zhao, Ying Sun, Shengnan Huang, Zhiyong Liu, Hui Feng
Journal: 3 Biotech 2022 v.12 no.4 pp. 104
Institution:College of Horticulture, Shenyang Agricultural University
Paper link:https://link.springer.com/article/10.1007/s13205-022-03160-z
41. [2021 IF 5.75] The Interaction Between CitMYB52 and CitbHLH2 Negatively Regulates Citrate Accumulation by Activating CitALMT in Citrus Fruit
Author:Shengchao Liu, Xincheng Liu, Bangrui Gou, Dengliang Wang, Chunrong Liu, Jun Sun, Xueren Yin , Donald Grierson , Shaojia Li and Kunsong Chen
Journal: Front. Plant Sci., 21 March 2022
Institution:College of Agriculture and Biotechnology, Zhejiang University
Paper link: https://www.frontiersin.org/articles/10.3389/fpls.2022.848869/full
42. [2021 IF 7.06] DEAP1 encodes a fasciclin-like arabinogalactan protein required for male fertility in rice
Author:Dan Zhou, Ting Zou, Kaixuan Zhang, Pingping Xiong, Fuxing Zhou, Hao Chen, Gongwen Li, Kaiyou Zheng, Yuhao Han, Kun Peng, Xu Zhang, Shangyu Yang, Qiming Deng, Shiquan Wang, Jun Zhu, Yueyang Liang, Changhui Sun, Xiumei Yu, Huainian Liu, Lingxia Wang, Ping Li, Shuangcheng Li
Journal: JIPB, First published: 29 April 2022
Institution:Sichuan Agricultural University
Paper link: https://onlinelibrary.wiley.com/doi/10.1111/jipb.13271
43. [2021 IF 6.48] Secretory lipid transfer protein OsLTPL94 acts as a target of EAT1 and is required for rice pollen wall development
Author:Yang Tao, Ting Zou, Xu Zhang, Rui Liu, Hao Chen, Guoqiang Yuan, Dan Zhou, Pingping Xiong, Zhiyuan He, Gongwen Li, Menglin Zhou, Sijing Liu, Qiming Deng, Shiquan Wang, Jun Zhu, Yueyang Liang, Xiumei Yu, Aiping Zheng, Aijun Wang, Huainian Liu, Lingxia Wang, Ping Li, Shuangcheng Li
Journal: Plant J, Volume108, Issue2 October 2021 Pages 358-377
Institution:Sichuan Agricultural University
Paper link:https://onlinelibrary.wiley.com/doi/epdf/10.1111/tpj.15443
44. [2021 IF 4.4] GmSAP5, a soybean A20/AN1 domain-containing stress-associated protein gene activated by GmAREB3, increases drought stress resistance in soybean by mediating ABA signaling
Author: Zehao Hou, Xiangzhan Zhang, Yimiao Tang, Taifei Yu, Lei Zheng, Jun Chen,Yongbin Zhou, Yongwei Liu, Ming Chen, Zhao-Shi Xu, Youzhi Ma
Journal: Crop J, 25 March 2022
Institution:Institute of Crop Sciences, Chinese Academy of Agricultural Sciences
Paper link: https://www.sciencedirect.com/science/article/pii/S2214514122000836?via%3Dihub
45. [2021 IF5.75] CmWRKY15-1 Promotes Resistance to Chrysanthemum White Rust by Regulating CmNPR1 Expression
Author: Ge Gao, Ruibing Jin, Di Liu, Xin Zhang, Xiaomei Sun, Pengfang Zhu and Hongyu Mao
Journal: Fronties in Plant Sciences, April 2022 | Volume 13 | Article 865607
Institution:College of Forestry, Shenyang Agricultural University
Paper link: https://www.frontiersin.org/articles/10.3389/fpls.2022.865607/full
46. [2021 IF10.15] Two chloroplast-localized MORF proteins act as chaperones to maintain tetrapyrrole biosynthesis
Author: Jiarui Yuan,Tingting Ma,Shuiling Ji,Boris Hedtke,Bernhard Grimm,Rongcheng Lin
Journal: New Phytologist, Volume235, Issue5 September 2022 Pages 1868-1883
Institution:Key Laboratory of Photobiology, Institute of Botany, Chinese Academy of Sciences
Paper link: https://nph.onlinelibrary.wiley.com/doi/10.1111/nph.18273
47. [2021 IF8.6] Rice OsUBR7 modulates plant height by regulating histone H2B monoubiquitination
and cell proliferation
Author: Yangyi Zheng, Sensen Zhang, Yanqiu Luo, Fuquan Li, Jiantao Tan, Bin Wang, Zhe Zhao, Huifang Lin, Tingting Zhang, Jianhong Liu, Xupeng Liu, Jingxin Guo, Xianrong Xie, Letian Chen, Yao-Guang Liu, and Zhizhan Chu
Journal: Plant Communications, Accepted Date: 8 July 2022
Institution:College of Life Sciences, South China Agricultural University
Paper link:https://www.sciencedirect.com/science/article/pii/S2590346222002449
48. [2021 IF12.085] The mycorrhiza-specific ammonium transporter ZmAMT3;1 mediates mycorrhiza-dependent nitrogen uptake in maize roots
Author: Jing Hui, Xia An, Zhibo Li, Benjamin Neuh?user, Uwe Ludewig, Xuna Wu, Waltraud X Schulze, Fanjun Chen, Gu Feng, Hans Lambers, Fusuo Zhang, Lixing Yuan
Journal: Plant Cell, 34(10):4066-4087
Institution:China Agricultural University
Paper link:https://pubmed.ncbi.nlm.nih.gov/35880836/
49. [2021 IF12.085] The RNA helicase UAP56 and the E3 ubiquitin ligase COP1 coordinately regulate alternative splicing to repress photomorphogenesis in Arabidopsis
Author: Yang Li, Yanxin Du, Junling Huai, Yanjun Jing, Rongcheng Lin
Journal: Plant Cell, 34(11):4191-4212
Institution:Key Laboratory of Photobiology, Institute of Botany, Chinese Academy of Sciences
Paper link:https://doi.org/10.1093/plcell/koac235
50. [2021 IF12.085] A SlCLV3-SlWUS module regulates auxin and ethylene homeostasis in low light–induced tomato flower abscission
Author: Lina Cheng , Ruizhen Li , Xiaoyang Wang , Siqi Ge , Sai Wang , Xianfeng Liu ,Jing He , Cai-Zhong Jiang , Mingfang Qi , Tao Xu , and Tianlai Li
Journal: Plant Cell, 34(11):4388-4408,2022
Institution:College of Horticulture, Shenyang Agricultural University
Paper link:https://academic.oup.com/plcell/advance-article/doi/10.1093/plcell/koac254/6668481?searchresult=1
51. [2021 IF 8.0] Transcription factors ABF4 and ABR1 synergistically regulate amylase-mediated starch catabolism in drought tolerance
Author: Yu Zhang, Jian Zhu, Madiha Khan, Yue Wang, Wei Xiao, Tian Fang, Jing Qu, Peng Xiao, Chunlong Li, Ji-Hong Liu
Journal: Plant Physiology, 14 September 2022
Institution:College of Horticulture and Forestry Sciences, Huazhong Agricultural University
Paper link: https://doi.org/10.1093/plphys/kiac428
52. [2021 IF 7.947] Metallochaperone OsHIPP9 is involved in the retention of cadmium and copper in rice
Author: Shuo Xiong, Xiaohang Kong, Guoqiang Chen, Lihong Tian, Dandan Qian, Zhen Zhu, and Le Qing Qu
Journal: Plant Cell and Environment , 2023
Institution:Key Laboratory of Plant Molecular Physiology, Institute of Botany, Chinese Academy of Sciences
Paper link: https://onlinelibrary.wiley.com/doi/10.1111/pce.14576
53. [2021 IF 4.927] Induction, Flavonoids Contents, and Bioactivities Analysis of Hairy Roots and True Roots of Tetrastigma hemsleyanum Dielset Gilg
Author: Hongzhen Wang, Anran Wang, Hanying Pu, Yuxin Yang, Zeyuan Ling, Haishun Xu,Juan Xu, Haizheng Yu and Xueqian Wu
Journal: Molecules 2023, 28, 2686
Institution:Zhejiang Agriculture and Forestry University
Paper link: https://doi.org/10.3390/molecules28062686
54. [2021 IF 8.0] The microRNA ppe-miR393 mediates auxin-induced peach fruit softening by promoting ethylene production
Author: Li Ma, Yingjie Zhao, Miaojin Chen, Yichen Li, Zhijun Shen, Yang Cao, Di Wu, Mingliang Yu, Donald Grierson, Yanna Shi, Kunsong Chen
Journal: Plant Physiology 2023
Institution:College of Agriculture and Biotechnology, Zhejiang University
Paper link: https://doi.org/10.1093/plphys/kiad182
55. [2021 IF 6.6] AtSWEET1 negatively regulates plant susceptibility to root-knot nematode disease
Author: Yuan Zhou, Dan Zhao , Yuxi Duan, Lijie Chen, Haiyan Fan, Yuanyuan Wang, Xiaoyu Liu, Li-Qing Chen, Yuanhu Xuan, Xiaofeng Zhu
Journal: Fronties in Plant Sciences 2023; 14: 1010348
Institution:Shenyang Agriculture University
Paper link: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9941640/
56. [2021 IF 8.6] Parallel evolution of two AIM24 protein subfamilies and their conserved functions in ER stress tolerance in land plants.
Author: Yanlong Guan, Guanxiao Chang, Jinjie Zhao, Qia Wang, Jiali Qin, Mengmeng Tang,Shuanghua Wang, Lan Ma, Jianchao Ma, Guiling Sun, Yun Zhou and Jinling Huang
Journal: Plant Communications 24, 100513, May 8 2023
Institution:Kunming Institute of Botany, Chinese Academy of Sciences
Paper link: https://www.cell.com/plant-communications/fulltext/S2590-3462(22)00360-1
57. [2021 IF 4.3] SlSWEET11b mediates sugar reallocation to regulate tomato stem morphogenesis.
Author: Jiaqi Sun, Lulu Lia, Xin Liu, Chaoyang Feng, Jing Jiang
Journal: Scientia Horticulturae 321 (2023) 112239
Institution:College of Horticulture, Shenyang Agricultural University
Paper link: https://doi.org/10.1016/j.scienta.2023.112239
58. [2022 IF 13.8] DGW1, encoding an hnRNP-like RNA binding protein, positively regulates grain size and weight by interacting with GW6 mRNA.
Author: Lingfeng Li, Jijin Li, Keke Liu, Chenglong Jiang, Wenhu Jin, Jiangkun Ye, Tierui Qin , Binjiu Luo, Zeyu Chen, Jinzhao Li, Fuxiang Lv, Xiaojun Li, Haipeng Wang, Jinghua Jin, Qiming Deng , Shiquan Wang , Jun Zhu, Ting Zou, Huainian Liu , Shuangcheng Li , Ping Li, Yueyang Liang.
Journal: Plant Biotechnology Journal Vol 2, 512-526,2024
Institution:Sichuan Agricultural University
Paper link: https://pubmed.ncbi.nlm.nih.gov/37862261/
59. [2022 IF 8.2] Integrated BSA-seq and RNA-seq analysis to identify candidate genes associated with nitrogen utilization efficiency (NUtE) in rapeseed (Brassica napus L.).
Author: Yunyou Nan, Yuyu Xie, Huiying He, Han Wu, Lixing Gao, Ayub Atif, Yanfeng Zhang, Hui Tian , Jing Hui, Yajun Gao
Journal: International Journal of Biological Macromolecules Volume 254, Part 1, January 2024, 127771
Institution:College of Natural Resources and Environment, Northwest A&F University
Paper link: https://www.sciencedirect.com/science/article/abs/pii/S0141813023046706
60. [2022 IF 5.5] Rapid and Highly Ef?cient Genetic Transformation and Application of Interleukin-17B Expressed in Duckweed as Mucosal Vaccine Adjuvant
Author: Xiao Tan, Shuang Chen, Yang Fang, Penghui Liu, Zhubin Hu, Yanling Jin, Zhuolin Yi, Kaize He ,Xing Li, Leyi Zhao, Hongning Wang,Hai Zhao.
Journal: biomolecules 2022, 12, 1881
Institution:Chengdu Institute of Biology, Chinese Academy of Sciences
Paper link: https://doi.org/10.3390/biom12121881
61. [2022 IF 4.5] Functional and Transcriptome Analysis Reveal Specific Roles of Dimocarpus longan DlRan3A and DlRan3B in Root Hair Development, Reproductive Growth, and Stress Tolerance
Author: Qilin Tian, Xiying Xie, Ruilian Lai, Chunzhen Cheng, Zihao Zhang, Yukun Chen, Xuhan Xu ,Yuling Lin and Zhongxiong Lai
Journal: plants 2024, 13, 480
Institution:Fujian Agriculture and Forestry University
Paper link: https://doi.org/10.3390/plants13040480
62. [2022 IF 11.4] SlBEL11 regulates ?avonoid biosynthesis, thus ?ne‐tuning auxin ef?ux to prevent premature fruit drop in tomato
Author: Xiufen Dong, Xianfeng Liu, Lina Cheng, Ruizhen Li, Siqi Ge, Sai Wang, Yue Cai,Yang Liu, Sida Meng, CaiZhong Jiang, ChunLin Shi, Tianlai Li, Daqi Fu, Mingfang Qi,Tao Xu
Journal: Journal of Integrative Plant Biology 2024
Institution:College of Horticulture, Shenyang Agricultural University
Paper link: https://doi.org/10.1111/jipb.13627
63. [2022 IF 4.3] Improving Sedum plumbizincicola genetic transformation with the SpGRF4–SpGIF1 gene and the self?excision CRE/LoxP system
Author: Yixin Zhang, Yanlan Mo,Hongxu Ren, Xiaotong Wu,Liyuan Han,Zhenyuan Sun, Wenzhong Xu
Journal: Planta (2024) 259:119
Institution:State Key Laboratory of Plant Diversity and Specialty Crops, Institute of Botany, Chinese Academy of Sciences
Paper link: https://doi.org/10.1007/s00425-024-04393-3
64. [2022 IF 暫無] SlSWEET10a negatively regulates sucrose transport in tomato fruit.
Author: Xinsheng Zhang, Jiaqi Sun, Xin Liu, Jing Jiang
Journal: Vegetable Research 2024, 4: e018
Institution: College of Horticulture, Shenyang Agricultural University
Paper link: https://doi.org/10.48130/vegres-0024-0018
65. [2022 IF 7.2] A novel three-layer module BoMYB1R1-BoMYB4b/BoMIEL1-BoDFR1 regulates anthocyanin accumulationin kale.
Author: Yang Liu, Hangbiao Jin, Yuting Zhang, Xin Feng, Yujia Dai,Pengfang Zhu
Journal: The Plant Journal (2024)
Institution: College of Forestry, Shenyang Agricultural University
Paper link: https://onlinelibrary.wiley.com/doi/full/10.1111/tpj.16881
66. [2022 IF 5.6] Overexpression of BnaC8.NRT2.1b improves nitrogen uptake efficiency in Brassica napus L.
Author: Yunyou Nan, Yuyu Xie, Ayub Atif, Derong Shi, Yanfeng Zhang, Hui Tian, Jing Hui, Yajun Gao
Journal: Industrial crops and products 219 (2024) 119058
Institution: College of Natural Resources and Environment, Northwest A&F University
Paper link:https://doi.org/10.1016/j.indcrop.2024.119058
67. [2022 IF 6.0] The dual role of casein kinase 1, DTG1, in regulating tillering and grain size in rice.
Author: Jijin Li, Dan Zhou, Deke Li, Gen Wang, Rui Qin, Chengqin Gong, Kang Chen, Yunqing Ton,Lingfeng Li, Keke Liu, Jiangkun Ye, Binjiu Luo, Chenglong Jiang, Haipeng Wang, Jinghua Jin,Qiming Deng, Shiquan Wang, Jun Zhu, Ting Zou, Shuangcheng Li, Ping Li, Yueyang Liang
Journal: The Crop Journal 2024
Institution:Sichuan Agricultural University
Paper link:https://doi.org/10.1016/j.cj.2024.07.016
68. [2022 IF 5.6] Involvement of isopropylmalate dehydratase in chloroplast development and acylsugar accumulation in tobacco.
Author: Hui Zhang , Qingxia Zheng , Guoyun Xu , Niu Zhai , Zefeng Li , Peipei Zhang , Pingping Liu,
Lifeng Jin , Huina Zhou
Journal: Industrial Crops & Products 222 (2024) 119543
Institution:Zhengzhou Tobacco Research Institute of CNTC
Paper link:https://doi.org/10.1016/j.indcrop.2024.119543
69. [2022 IF 8.3] Ultraviolet attenuates centromere-mediated meiotic genome stability and alters gametophytic ploidy consistency in ?owering plants.
Author: Huiqi Fu, Jiaqi Zhong, Jiayi Zhao, Li Huo, ChongWang, Dexuan Ma,Wenjing Pan, Limin Sun, Ziming Ren, Tianyi Fan, ZeWang, WenyiWang, Xiaoning Lei, Guanghui Yu, Jing Li, Yan Zhu,Danny Geelen,Bing Liu
Journal: New Phytologist (2024)
Institution:College of Life Sciences, South-Central Minzu University
Paper link:https://doi.org/10.1111/nph.19978
70. [2022 IF 2.4] Molecular Cloning and Functional Characterization of a Cytochrome P450 Enzyme (SaCYP736A167) Promoter from Santalum album.
Author: Haifeng Yan, Yueya Zhang, Rongchang Wei , Lihang Qiu , Huiwen Zhou , Faqian Xiong ,Guohua Ma
Journal: Forests 2024, 15, 1705.
Institution:South China Botanical Garden,Chinese Academy of Sciences
Paper link:https://doi.org/10.3390/f15101705
71. [2022 IF 5.6] Establishment of clary sage (Salvia sclarea L.) regeneration system and development of glyphosate-tolerant clary sage plants through Agrobacterium-mediated genetic transformation.
Author: Mengjuan Hou, Hui Kong, Wenwen Xia , Jin Li, Jianbo Zhu
Journal: Industrial Crops & Products 222 (2024) 119745
Institution:Key Laboratory of Agricultural Biotechnology, College of Life Science, Shihezi University
Paper link:https://doi.org/10.1016/j.indcrop.2024.119745
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