6.5 kD單條蛋白Marker

●產品編號及規(guī)格：

● 儲存、效期及運輸：

  -20℃保存；有效期一年；濕冰運輸。

● 產品簡介：

   本產品包含一種已知分子量的標準蛋白質，分子量大小為6.5 kD，可以用來判斷多肽電泳時蛋白的分子量（變性還原電泳）。產品配套非變性電泳上樣緩沖液，可用于粗略判斷多肽非變性電泳時條帶的大小(非變性非還原電泳)。

6.5 kD單條蛋白Marker 以干粉狀態(tài)提供。以每次使用配制好的溶液10 μl（0.2 μg/μl）計算，該產品可以大約使用500次。

● 使用說明：

一. 配制10 mg/ml 6.5 kD蛋白Marker母液：

1 mg 粉末溶于100 μl超純水中，徹底溶解。此溶液-20℃貯存，有效期一年。

二. 按照下表配制Marker溶液

注：由于2×Tricine多肽上樣緩沖液（變性，還原）中DTT含量會隨貯存時間延長而降低，變性還原電泳可以選擇補加DTT。

2.1  取配制好的溶液徹底混勻后，立即上樣電泳，每次上樣10 μl。

2.2  電泳結束后，染色，觀察結果。

注：6.5 kD蛋白電泳要使用Tricine系統(tǒng)電泳（貨號：RTD6120和RTD6121），如使用經典Laemmli系統(tǒng)會導致片段不可見。

三. 實驗示例：

使用蛋白Marker產品發(fā)表部分文章列表：

1. [2021 IF=5.5] Garviecin LG34, a novel bacteriocin produced by Lactococcus garvieaeisolated from traditional Chinese fermented cucumber.

Author: Yurong Gao, Dapeng Li, Shan Liu, Liyuan Zhang

Marker: RTD6110，彩虹超低分子量蛋白Marker（1.2-45 kD）

Journal: Food Control 50 (2015) 896e900

Institution：Key Laboratory of Veterinary Medicine of Heilongjiang Bayi Agricultural University

Paper link：http://dx.doi.org/10.1016/j.foodcont.2014.10.040

2. [2021 IF=5.5] Purification and partial characterization of M1-UVs300, a novel bacteriocin produced by   isolated from fermented sausage Lactobacillus plantarum.

Author: Yu An, Ying Wang, Xiaoyue Liang, Huaxi Yi, Zhaohang Zuo, Xiaoxi Xu, Dongjie

Zhang, Changqing Yu, Xue Han

Marker: RTD6110，彩虹超低分子量蛋白Marker（1.2-45 kD）

Journal: Food Control  Volume 81, November 2017, Pages 211-217

Institution：Key Laboratory of Veterinary Medicine of Heilongjiang Bayi Agricultural University

Paper link：https://doi.org/10.1016/j.foodcont.2017.05.030

3. [2021 IF=5.75] The Verticillium dahliae SnodProt1-Like Protein VdCP1 Contributes to Virulence and Triggers the Plant Immune System.

Author: Yi Zhang, Yuhan Gao, Yingbo Liang, Yijie Dong, Xiufen Yang, Jingjing Yuan and Dewen Qiu

Marker: RTD6110，彩虹超低分子量蛋白Marker（1.2-45 kD）

Journal: Frontiers in Plant Science.  Volume 81, November 2017, Pages 211-217

Institution：Institute of Plant Protection, Chinese Academy of Agricultural Sciences

Paper link：https://doi.org/10.1093/jxb/ery351

4. [2021 IF=2.99] Purifcation, characterization, and action mechanism of plantaricin DL3, a novel bacteriocin against Pseudomonas aeruginosa produced by Lactobacillus plantarum DL3 from Chinese Suan Tsai.

Author: Xinran Lv,  Yang Lin ,Yu Jie, Mengtong Sun, Bolin Zhang,Fengling Bai,Hongfei Zhao,Jianrong Li

Marker: RTD6127，超低分子量蛋白Marker IV（3.3-45 kD）

Journal: European Food Research and Technology. Vol 244, pages 323–331 (2018)

Institution：College of Biological Science and Biotechnology, Beijing Forestry University

Paper link：https://link.springer.com/article/10.1007/s00217-017-2958-3

5. [2021 IF=6.99] Verticillium dahliae Alt a 1-like protein PevD1 targets cotton PR5-like protein and contributes to fungal infection.

Author: Yi Zhang, Yuhan Gao, Yingbo Liang, Yijie Dong, Xiufen Yang, Dewen Qiu

Marker: RTD6110，彩虹超低分子量蛋白Marker （1.2-45 kD）

Journal: Journal of Experimental Botany, Vol 70, Issue 2, 15 January 2019，613-626

Institution：Institute of Plant Protection, Chinese Academy of Agricultural Sciences

Paper link：https://doi.org/10.1093/jxb/ery351

6. [2021 IF=11.277] An  Alternative  Splicing  Variant  of  PtRD26  Delays  Leaf  Senescence by Regulating Multiple NAC Transcription Factors in Populus.

Author: Hou-Ling Wang,Yi Zhang,Ting Wang, Qi Yang,Yanli Yang,Ze Li, Bosheng Li, Xing Wen, Wenyang Li, Weilun Yin, Xinli Xia, Hongwei Guo, and Zhonghai Li

Marker: RTD6110，彩虹預染超低分子量蛋白（1.2-45 kD）

Journal: Plant Cell. Volume 33, Issue 5, May 2021, Pages 1594–1614

Institution：Beijing Advanced Innovation Center for Tree Breeding by Molecular Design, Beijing Forestry University

Paper link：https://doi.org/10.1093/plcell/koab046

7. [2021 IF=2.89] Rapid and sensitive detection ofStaphylococcus aureus and Klebsiella pneumonia based on bacitracin-modified Fe3O4@PDA magnetic beads combined with matrix-assisted laser desorption ionization-time of flight mass spectrometry.

Author: Feng Qin, Shujuan Wang, Mingxia Gao  and  Xiangmin Zhang

Marker: RTD6110，彩虹預染超低分子量蛋白（1.2-45 kD）

Journal: Analytical Methods. 2021,13, 2804-2811

Institution：Department of Chemistry, Fudan University

Paper link：https://pubs.rsc.org/en/content/articlelanding/2021/ay/d1ay00614b

8. [2021 IF=3.74] Cloning, expression, and function of ferritins in the tick Haemaphysalis flava.

Author: Yu Zhao, Lei Liu, Jin-Bao Liu, Cong-Ying Wu, De-Yong Duan, Tian-Yin Cheng

Marker: RTD6142 高分子量非變性電泳蛋白質Marker II（45-669 kD）

Journal: Ticks and Tick-borne Diseases. Volume 13, Issue 2, March 2022, 101892

Institution：College of Veterinary Medicine, Hunan Agricultural University,

Paper link：https://linkinghub.elsevier.com/retrieve/pii/S1877959X21002454

9. [2021 IF=0.886] Purification and Characterization of an Intracellular β-Glucosidase from the Protoplast Fusant of Aspergillus oryzae and Aspergillus niger

Author: F.M. Zhu, B. Du, H.S. Gao, C.J. Liu, and J. Lia

Marker: RTD6109 高分子量蛋白質Marker（43-200 kD）

Journal: Applied Biochemistry and Microbiology, 2010, Vol. 46, No. 6, pp. 626–632

Institution：College of Food Science and Technology, Hebei Normal University of Science and Technology

Paper link：https://link.springer.com/article/10.1134/S0003683810060116

10. [2021 IF=4.374] Effect of Fuzhuan brick-tea addition on the quality and antioxidant activity of skimmed set-type yoghurt.

Author: Dong Liu

Marker: RTD6103 低分子量蛋白質Marker II（14.4-116 kD）

Journal: International Journal of Dairy Technology, Vol 71 March 2018 Pages 22-33

Institution：Department of Food Processing Technology, Anqing Vocational & Technical College

Paper link：https://doi.org/10.1111/1471-0307.12395

11. [2021 IF=3.7] Smart delivery of poly-peptide composite for effective cancer therapy

Author: Xin Yuan, Yingzhou Qin, Qingmei Tian, Cuijuan Liu, Xiangzhou Meng, Bo Qie, Fan Gao, Ying Huang, Guanghui Xu, Guang Yang, Yimin Zhu

Marker: Protein Marker

Journal: Biomedical Materials, 2022 Jan 24;17(2)

Institution:School of Nano-Tech and Nano-Bionics, University of Science and Technology of China

Paper link：https://pubmed.ncbi.nlm.nih.gov/34996052/